Imaging Cameras & Sensors – Wavefront Sensors
Plug and Play Quantitative Phase Imaging Camera
High-Performance Plug-and-Play Quantitative Phase Imaging Camera for Label-Free Microscopy and Material Metrology
The Plug-and-Play Quantitative Phase Imaging Camera – SID4Bio is an easy-to-integrate microscope imaging solution that delivers instant, label-free visualisation of transparent specimens. Designed for quantitative phase imaging (QPI), this compact camera works seamlessly with standard optical microscopes, capturing both phase and intensity data simultaneously with excellent sensitivity and stability. Ideal for life science, biomedical research, and material inspection, it enables precise measurement of optical path differences, refractive index, cell mass, and structural features. Its robust design, intuitive setup, and high-quality data acquisition make it a powerful choice for laboratories and industrial environments seeking high-resolution, artefact-free phase imaging without extensive configuration.
Understanding Plug and Play Quantitative Phase Imaging Camera
The plug-and-play quantitative phase imaging camera uses advanced wavefront sensing technology to measure phase shifts in light after it passes through or reflects off a sample, revealing optical path differences that correlate with refractive index and thickness. It captures both intensity and phase images in one shot, avoiding artefacts from multiple exposures or mechanical scanning. The system is compatible with white-light and laser illumination, supports a broad spectral range from visible to near-IR, and interfaces easily via Gigabit Ethernet. Its compact footprint and simple microscope integration make it suitable for diverse imaging workflows across research and industrial applications.
Technical Specifications
| Specification | Value |
| Wavelength Range | 400 – 1100 nm |
| Phase & Intensity Sampling | 416 × 360 pixels |
| Lateral Resolution | Down to 0.2 µm (with ×120 magnification) |
| Phase Resolution | < 2 nm RMS |
| Acquisition Rate | 10 frames per second |
| Dry Mass Resolution | < 0.5 pg |
| Microscope Interface | C-mount |
| Data Interface | Gigabit Ethernet |
| Dimensions (W×H×D) | ~73 × 71 × 90 mm |
| Weight | ~450 g |
Key Features and Advantages
True Quantitative Phase Imaging
This camera captures both phase and intensity information in a single acquisition, enabling accurate optical path difference measurement without artefacts from multi-exposure methods.
Compact Plug-and-Play Design
Its robust, lightweight form factor ensures quick integration with any standard optical microscope via a C-mount interface, simplifying setup and reducing downtime.
High Sensitivity and Resolution
With phase resolution under 2 nm RMS and the ability to resolve lateral features down to 0.2 µm (at high magnification), this system provides detailed structural and optical insights essential for advanced imaging tasks.
Broad Spectral Compatibility
Operating across the visible to near-infrared range (400 – 1100 nm), it supports a wide variety of illumination sources, including white light and lasers for versatile imaging conditions.
Real-Time Data Acquisition
An acquisition rate of 10 fps enables dynamic phase imaging and time-lapse studies, supporting live cell monitoring and other evolving phenomena.
Dry Mass Measurement
The high dry mass resolution (< 0.5 pg) allows quantification of cell and sample mass distribution, useful in cell biology, growth studies, and drug response analysis.
Robust Data Interface
Gigabit Ethernet connectivity supports reliable, high-speed data transfer and remote operation over standard network infrastructure.
Non-Invasive, Label-Free Imaging
Phase imaging avoids the need for stains or fluorescent markers, ensuring sample integrity, reducing preparation time, and eliminating phototoxicity.
Applications Across Industries
Life Sciences and Cell Biology
Quantitative phase imaging enables live, label-free monitoring of cellular morphology, growth, and dry mass evolution, crucial for cytotoxicity assays, cell cycle studies, and stem cell research.
Biomedical Research and Diagnostics
Measurement of optical path differences and refractive index variations supports tissue inspection, morphology studies, and early detection of disease-related changes without stains or dyes.
Material Science and Metrology
Accurate phase data assists in assessing thin films, refractive index mapping, surface microstructure, and defect detection in industrial materials and optical components.
Pharmaceutical and Drug Development
Time-lapse phase imaging tracks cell response to compounds over time, enabling high-content screening and quantitative analysis in preclinical studies.
Microscopy Enhancements
Phase imaging augments traditional brightfield and DIC microscopy, revealing details invisible to intensity-only imaging and enabling multimodal workflows.
Education and Research Institutions
Universities and labs use quantitative imaging to teach and study advanced optics, cell behaviour, and material properties with a hands-on imaging platform.
Why Choose United Spectrum Instruments?
United Spectrum Instruments offers expert support, local integration services, and technical training for advanced imaging systems like the plug-and-play quantitative phase imaging camera. Our team assists with setup, calibration, and workflow optimisation, ensuring seamless incorporation into research and industrial environments. With responsive after-sales support and deep understanding of microscopy and metrology applications, we help customers unlock the full potential of quantitative phase imaging technology. Partnering with United Spectrum Instruments means reliable performance, application expertise, and ongoing service tailored to your scientific and industrial imaging needs.
FAQs
What is quantitative phase imaging?
Quantitative phase imaging measures phase shifts of light caused by samples, enabling label-free visualisation of structure, thickness, and refractive index.
Does this camera require special illumination?
No; it works with common white-light sources and lasers across 400 – 1100 nm.
What types of samples can be imaged?
Transparent specimens like live cells, thin films, and optical materials benefit most from phase imaging
Can it be used with any microscope?
Yes; the C-mount interface allows integration with standard optical microscopes.
Is real-time imaging supported?
Yes; the camera supports up to 10 fps for live phase imaging studies.
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FAQs
What is quantitative phase imaging?
Quantitative phase imaging measures phase shifts of light caused by samples, enabling label-free visualisation of structure, thickness, and refractive index.
Does this camera require special illumination?
No; it works with common white-light sources and lasers across 400 – 1100 nm.
What types of samples can be imaged?
Transparent specimens like live cells, thin films, and optical materials benefit most from phase imaging
Can it be used with any microscope?
Yes; the C-mount interface allows integration with standard optical microscopes.
Is real-time imaging supported?
Yes; the camera supports up to 10 fps for live phase imaging studies.

