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Scientific CMOS (sCMOS) Quantitative Phase Imaging Camera

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Sid4 sc8 quantitative phase imaging camera

High-Sensitivity Scientific sCMOS Quantitative Phase Imaging Camera for Label-Free Microscopy and Material Metrology

The Scientific CMOS (sCMOS) Quantitative Phase Imaging Camera- SID4 sC8 delivers breakthrough imaging capability for label-free, high-precision phase measurement in both life science and material inspection applications. Designed around advanced sCMOS sensor technology, this camera captures true quantitative phase and intensity information in a single shot, enabling non-invasive imaging of transparent specimens and precise surface characterization. With a compact, plug-and-play form factor and compatibility with standard microscopes and acquisition software, researchers can seamlessly integrate powerful phase imaging into existing workflows. Ideal for live cell analysis, refractive index mapping, and optical metrology, this system expands imaging possibilities with sub-nanometric sensitivity and real-time performance.

The quantitative phase imaging camera uses sCMOS sensor technology to capture both phase shift and intensity values from a specimen in a single image acquisition, enabling true quantitative phase imaging (QPI) without labels or staining. This is achieved using Phasics’ patented wavefront sensing techniques that measure the optical path difference created when light passes through or reflects off a transparent sample. The system operates across a broad visible to near-infrared spectral range, delivering high sensitivity with low noise and rapid frame rates. Its compact design and USB3.0 interface make it easy to connect to microscopes, workstations, and imaging setups for both scientific research and industrial metrology.

ParameterSpecification
Imaging SensorScientific CMOS (sCMOS)
Sensor Resolution2048 × 2048 pixels (≈4.2 MP)
Pixel Size6.5 µm × 6.5 µm
Frame RateUp to 30 fps (full resolution)
Phase Sensitivity< 2 nm RMS (optical path difference)
Phase Measurement Accuracy±5 nm (typical)
Spatial ResolutionLimited by microscope optics (down to diffraction limit)
Wavelength Range400 – 1050 nm
Dynamic Phase Range> 20 µm optical path difference
Measurement ModeSingle-shot quantitative phase + intensity
Acquisition TypeReal-time, full-field phase imaging
Bit Depth16-bit
Noise (Read Noise)< 2 electrons RMS
InterfaceUSB 3.0
Optical MountC-mount
Compatible Objectives5× to 100× (NA dependent)
Minimum Detectable Thickness Change~1–2 nm (material dependent)
Refractive Index Resolution~1 × 10⁻⁴
Field of ViewSensor-limited; objective dependent
Calibration RequirementFactory calibrated; software-assisted recalibration
Software CompatibilityMicro-Manager, MetaMorph, custom SDK
Operating Temperature+10 °C to +40 °C
Power SupplyUSB-powered / external 12 V (depending on configuration)
Camera DimensionsCompact industrial camera housing
System Weight< 1.5 kg

Advanced sCMOS Sensor Technology

Equipped with a scientific CMOS sensor, this camera combines low noise, high quantum efficiency, and fast frame rates, ideal for high-resolution imaging and dynamic phase measurement.

Single-Shot Phase and Intensity Capture

Simultaneously records precise phase and intensity data in one frame, enabling accurate and artefact-free quantitative phase imaging without the need for multiple exposures.

Sub-Nanometric Phase Sensitivity

Capable of resolving optical path differences with sub-nanometric accuracy (< 2 nm RMS), making it suitable for detailed thickness or refractive index mapping in transparent samples. phasics.com

Plug-and-Play Integration

Compact form factor and standard interfaces (USB3.0, C-mount) simplify integration with existing optical microscopes or imaging systems without complex setup.

Broad Spectral Range

Operates over a 400–1050 nm wavelength range, supporting flexibility in illumination and compatibility with various contrast methods.

Real-Time Processing

Supports real-time phase reconstruction and live imaging workflows at rates up to ~10 Hz (full resolution), enabling dynamic studies such as time-lapse microscopy.

Software Compatibility

Works with popular acquisition software such as Micro-Manager, MetaMorph, and NIS-Elements, allowing familiar control and analysis environments.

Non-Invasive, Label-Free Imaging

Unlike fluorescence or staining techniques, quantitative phase imaging is non-destructive and avoids phototoxicity—ideal for live cell applications.

Versatile Measurement Modes

Unlocks applications in refractive index mapping, laser damage threshold analysis, surface topography, and biological morphology studies.

Life Sciences and Biomedical Research

Quantitative phase imaging allows label-free monitoring of live cells and tissues, measuring morphology, dry mass, and dynamic behaviour without staining. It supports studies such as cell cycle analysis, cytotoxicity screening, and stem cell research with high contrast and minimal artefacts.

Material Science and Metrology

The camera provides precise refractive index mapping, surface topography, and transparent material inspection. Researchers can analyse thin films, optical waveguides, nanostructures, and stress patterns with quantitative phase data for material characterisation and quality control.

Microscopy and Imaging Platforms

Quantitative phase imaging enhances traditional microscopy workflows by adding phase measurement capability to brightfield and fluorescence setups, enabling multimodal imaging with comprehensive datasets for detailed research.

Optics and Photonics Testing

Used for optical component testing such as lens surface profiling, metasurface analysis, and wavefront measurements, delivering highly accurate phase information critical to advanced optical manufacturing.

Semiconductor and Nanotechnology

Phase imaging can reveal sub-micrometric refractive index variations in semiconductor structures, aiding defect detection, waveguide metrology, and nano-feature characterisation.

Environmental and Biomedical Diagnostics

Enables label-free morphology studies for blood cells, microorganisms, and tissues, supporting diagnostics without traditional dyes or reagents.

Education and Research Institutions

Provides a versatile imaging tool for teaching advanced optics, microscopy, and quantitative imaging techniques across multidisciplinary research.

United Spectrum Instruments is your trusted partner for advanced scientific imaging solutions in India, offering expert guidance, local support, and seamless integration of high-performance systems like quantitative phase imaging cameras. We deliver tailored configuration, installation, and training services to ensure your laboratory or production workflow achieves maximum performance and reliability. Our team understands the precise requirements of research, microscopy, and metrology environments and provides responsive support throughout the system lifecycle. With United Spectrum Instruments, you get world-class imaging technology backed by industry expertise and dependable service.

FAQs

Quantitative phase imaging measures the phase shift of light through a sample, revealing optical path differences related to refractive index and thickness—without staining or labels.

Yes, it integrates easily with conventional optical microscopes via a C-mount interface.

Transparent or semi-transparent specimens such as live cells, thin films, optical waveguides, and biological tissues are ideal.

Yes, it supports real-time processing at up to ~10 Hz at full resolution, enabling live observation of dynamic processes

No; it is compatible with common imaging software like Micro-Manager and MetaMorph for acquisition and analysis.

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FAQs

Quantitative phase imaging measures the phase shift of light through a sample, revealing optical path differences related to refractive index and thickness—without staining or labels.

Yes, it integrates easily with conventional optical microscopes via a C-mount interface.

Transparent or semi-transparent specimens such as live cells, thin films, optical waveguides, and biological tissues are ideal.

Yes, it supports real-time processing at up to ~10 Hz at full resolution, enabling live observation of dynamic processes

No; it is compatible with common imaging software like Micro-Manager and MetaMorph for acquisition and analysis.

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